Evaluation of protein extraction methods in fingerlings of chame (Dormitator latifrons) for proteomic study
DOI:
https://doi.org/10.36097/rsan.v1i55.2525Keywords:
Dormitator latifrons, fingerlings, quantification, protein profile, proteomicsAbstract
The chame (Dormitator latifrons) is a freshwater fish with aquaculture potential in Latin America. Its production is limited to the use of juveniles captured from the wild. It has been possible to reproduce this species in captivity from broodstock obtained from the natural environment, although in most cases, the process fails in the passage from prolarvae to larvae due to lack of food that contains the necessary nutritional requirements, especially proteins such as some of the main compounds needed by fish. The objective of the research was to evaluate two protein extraction protocols from Dormitator latifrons fingerlings. Fingerling samples were taken from the natural environment, sacrificed by immersion in liquid N2 and stored at -80°C until protein extraction. This work compares the protein concentration and the protein profile, obtained by using two extraction buffers: a traditional RIPA; and ammonium bicarbonate, a volatile salt compatible with mass spectrometry. As a result, a better protein profile is shown in the samples treated with ammonium bicarbonate, which is why this protocol is concluded and recommended for future investigations of the global proteome, by means of mass spectrometry.
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Abdo-de la Parra, M., Rodríguez-Montes de Oca, G. A., Rodríguez-Ibarra, L. E., Domínguez-Jiménez, P., Román-Reyes, J. C., Velasco-Blanco, G., & Ibarra-Castro, L. (2017). Composición proximal y perfil de aminoácidos de estadios tempranos del pargo flamenco Lutjanus guttatus. Revista de biología marina y oceanografía, 52(2), 325-332. http://dx.doi.org/10.4067/S0718-19572017000200011.
Agualsaca, O. (2015). Adaptación de chame (Dormitator latifrons) sometido a cautiverio utilizando cuatro niveles de detritus y balanceado en su alimentación. [Tesis de grado, Universidad de las Fuerzas Armadas]. Repositorio Institucional ESPE. http://repositorio.espe.edu.ec/xmlui/handle/21000/9692
Badillo, D., Zaragoza, F., Vega, F., López, J., Herrera S., Cueto, L., & Guerrero, S. (2018). Requerimiento de proteína y lípidos para el crecimiento de juveniles del pez nativo Dormitator latifrons (Richardson, 1844). Ecosistemas y recursos Agropecuarios, 5(14), 345-351. https://doi.org/10.19136/era.a5nl4.1554.
Badillo, D., Musin, G., Palma, D., Guerrero, S., Chong, O. & Vega, F (2021). Total or partial replacement of fishmeal with soybean meal in the diet of the Pacific fat sleeper (Dormitator latifrons) juveniles. Latin American Journal of Aquatic Research, 49, 40-47. https://doi.org/10.3856/vol49-issue1-fulltext-2564.
Bradford, M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254. https://doi.org/10.1006/abio.1976.9999.
Cedeño, J. (2013). Alimentación del chame (Dormitator latifrons) con bovinaza y balanceado para mejorar la productividad. Investigación y Saberes, 2(2), 59-64. http://revistasdigitales.utelvt.edu.ec/revista/index.php/investigacion_y_saberes/article/view/43.
Casado, J., Rodríguez, I., Carpinteiro, I., Ramil, M., & Cela, R. (2013). Gas chromatography quadrupole time-of-flight mass spectrometry determination of benzotriazole ultraviolet stabilizers in sludge samples. Journal of Chromatography A, 1293(7), 126–132. https://doi.org/10.1016/j.chroma.2013.03.050.
Chicano, E. (2010). Análisis proteómicos del desarrollo larvario del lenguado senegalés (S. senegalensis) y anomalías durante su desarrollo [Tesis Doctoral, Universidad de Córdoba]. Archivo Digital de la Universidad de Córdoba. https://dialnet.unirioja.es/servlet/tesis?codigo=55374
Forné, I., Abián, J., Cerdà, J., 2010. Fish proteome analysis: model organisms and nonsequenced species. Proteomics 10, 858–872. https://doi.org/10.1002/pmic.200900609
Guajardo, L. (2005). Estudio de la proteómica nuclear del pez Cyprinus carpio durante el proceso de aclimatización estacional. [Tesis de grado, Universidad Austral de Chile, Facultad de Ciencias] Tesis Electrónicas UAch. https://n9.cl/wtcfd.
Laemmli, U. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685. https://doi.org/10.1038/227680a0.
Li, Y., Xie, W., & Li, Q. (2016b). Understanding the lipopolysaccharide induced liver proteome changes and identification of immune genes in Lampetra morii. Aquac. Fish, 1, 9–14. https://doi.org/10.1016/j.aaf.2016.09.002.
Liu, R., Ruan, T., Wang, T., Song, S., Guo, F., & Jiang, G., (2014). Determination of nine benzotriazole UV stabilizers in environmental water samples by automated on-line solid phase extraction coupled with high-performance liquid chromatographytandem mass spectrometry. Talanta, 120, 158–166. https://doi.org/10.1016/j. talanta.2013.10.041.
López, V., Rodríguez, G., Galavíz, M., Román, C., Medina, E., Dabrowski, K., & Haws, M. (2015). Descripción histológica comparativa del desarrollo del sistema digestivo y visual de larvas de chame Dormitator latifrons (Pisces: Eleotridae). Latin-American Journal of Aquatic Research, 43(3): 484-494. https://doi.org/10.3856/vol43-issue3-fulltext-10.
Montenegro, C., & Vallejo, A. (2015). Estudio del potencial acuícola del chame (Dormitator latifrons), en la vereda el olivo, municipio de Arboleda Berruecos, departamento de Nariño, Colombia [Tesis de Grado, Universidad de la Costa]. Repositorio Agrosavia. https://repository.agrosavia.co/bitstream/handle/20.500.12324/36602/Ver_Documento_36602.pdf?sequence=4&isAllowed=y
Nissa, M., Pinto, N., Parkar, H., Gosvami, M., & Srivastava, S. (2021). Proteomics in fisheries and aquaculture: An approach for food security. Food Control, 127, 108-125. https://doi.org/10.1016/j.foodcont.2021.108125.
Rodríguez, G., Medina, E., Velázquez, J., López, V., Román, J., Dabrowski, K., & Haws, M. (2012). Producción de larvas de chame (Dormitator latifrons, Pisces: Eleotridae) usando GnRHa and LHRHa. Revista Colombiana de Ciencias Pecuarias, 25(3), 422-429. https://www.redalyc.org/pdf/2950/295024923010.pdf
Zambrano, G. (2014). Análisis de la producción y comercialización del chame (Dormitator latifrons) en el Ecuador: provincia de Manabí cantón Chone período 2010-2013 [Tesis de Grado, Universidad de Guayaquil]. Repositorio Institucional de la Universidad de Guayaquil. http://repositorio.ug.edu.ec/handle/redug/9623
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Copyright (c) 2023 Agustin Norberto Zambrano Ostaiza, Elisa Espinosa López, Guadalupe Gómez Baena, Juan Manuel Vera Delgado
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