Evaluation of protein extraction methods in fingerlings of chame (Dormitator latifrons) for proteomic study
DOI:
https://doi.org/10.36097/rsan.v1i55.2525Keywords:
Dormitator latifrons, fingerlings, quantification, protein profile, proteomicsAbstract
The chame (Dormitator latifrons) is a freshwater fish with aquaculture potential in Latin America. Its production is limited to the use of juveniles captured from the wild. It has been possible to reproduce this species in captivity from broodstock obtained from the natural environment, although in most cases, the process fails in the passage from prolarvae to larvae due to lack of food that contains the necessary nutritional requirements, especially proteins such as some of the main compounds needed by fish. The objective of the research was to evaluate two protein extraction protocols from Dormitator latifrons fingerlings. Fingerling samples were taken from the natural environment, sacrificed by immersion in liquid N2 and stored at -80°C until protein extraction. This work compares the protein concentration and the protein profile, obtained by using two extraction buffers: a traditional RIPA; and ammonium bicarbonate, a volatile salt compatible with mass spectrometry. As a result, a better protein profile is shown in the samples treated with ammonium bicarbonate, which is why this protocol is concluded and recommended for future investigations of the global proteome, by means of mass spectrometry.
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Copyright (c) 2023 Agustin Norberto Zambrano Ostaiza, Elisa Espinosa López, Guadalupe Gómez Baena, Juan Manuel Vera Delgado
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